1. Sitong Liu#, Rui Wei#, Hongyang Liu, Ruiqi Liu, Pengxiao Li, Xiaoyu Zhang, Wei Wei, Xiaodong Zhao, Xiaomeng Li, Yang Yang, Xueqi Fu*, Kang Zou*. Analysis of Chromatin Accessibility in p53 Deficient Spermatogonial Stem Cells for High Frequency Transformation into Pluripotent State. Cell Proliferation. 2022. DOI: 10.1111/cpr.13195 2. Rui Wei#, Xiaoyu Zhang#, Yihui Cai, Hongyang Liu, Bingyuan Wang, Xiaodong Zhao, Kang Zou*. Busulfan Suppresses Autophagy in Mouse Spermatogonial Progenitor Cells via mTOR of AKT and p53 Signaling Pathways. Stem Cell Reviews and Reports. 2020. 16(6):1242-1255 3. Dan-Chen Zhang, Rong Chen, Yi-Hui Cai, Jing-Jing Wang, Chang Yin, Kang Zou*, Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43. Zoological Research. 2020. 41(2): 1-5. 4. Qin Xia, Danchen Zhang, Jingjing Wang, Xiaoyu Zhang, Weixiang Song, Rong Chen, Hua Li, Wenhai Xie*, Kang Zou*. Androgen Indirectly Regulates Gap Junction Component Connexin 43 via Wilms Tumor-1 in Sertoli Cells. Stem Cells and Development. 2020. 29 (3), 169-176. 5. Yihui Cai, Jingjing Wang, Kang Zou*. The Progresses of Spermatogonial Stem Cells Sorting using Fluorescence-activated Cell Sorting. Stem Cell Reviews and Reports. 2020;16(1):94-102.doi: 10.1007/s12015-019-09929-9. 6. Weixiang Song , Xinglong Shi , Qin Xia , Min Yuan, Jiaxi Liu, Kunying Hao, Yinjuan Qian, Xiaodong Zhao*, Kang Zou*. PLZF suppresses differentiation of mouse spermatogonial progenitor cells via binding of differentiation associated genes. Journal of Cellular Physiology.2020. 235(3): 3033-3042. 7. Yizhuo Sun, Sitong Liu, XM Li*, Kang Zou*. Progress in in vitro culture and gene editing of porcine spermatogonial stem cells.Zoological Research. 2019. 40(5): 343-348. 8. Jingjing Wang# , Jinmei Li# , Wei Xu , Qin Xia , Yunzhao Gu , Weixiang Song , Xiaoyu Zhang , Yang Yang , Wei Wang , Hua Li , Kang Zou*.Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory Pattern. Cell Communication and Signaling. 2019. 17(1), 57. 9. Xiaoyu Zhang, Rui Wei, Yizhuo Sun, Qin Xia, Wenhai Xie, Hongfei Song, Wei Wang, Kang Zou*. AKT3 is a pivotal molecule of CDH22 and GFRA1 signal pathways regulating Self-Renewal in Female Germline Stem Cells. Stem Cells. 2019;37:1095–1107. 10. Xiaoyu Zhang, Qin Xia, Rui Wei, Hongfei Song, Jiaqi Mi, Zhaoyu Lin, Yang Yang, Zijie Sun and Kang Zou*, Melatonin protects spermatogonia from the stress of chemotherapy and oxidation via eliminating reactive oxidative species. Free Radical Biology and Medicine. 2019. 12;137:74-86. 11. Kang Zou#, Jian Wan#, Haiwei Bi, Yabin Zhang, Xueli Tian, Ning Tian, Wanyun Ma* and Ji Wu*, Comparison of different in vitro differentiation conditions for murine female germline stem cells. Cell Proliferation. 2019. Jan;52(1): e12530. doi: 10.1111/cpr.12530. 12. Xiaoyu Zhang, Yang Yang, Qin Xia, Hongfei Song, Rui Wei, Jingjing Wang and Kang Zou*, Cadherin 22 participates in the self-renewal of mouse female germ line stem cells via interaction with JAK2 and ß-catenin. Cellular and Molecular Life Sciences. 2018.4, 75(7): 1241-1253. 13. Xiaoyu Zhang, Yue Peng and Kang Zou*, Germline Stem Cells, a Useful Tool for Therapeutic Cloning. Current Stem Cell Research and Therapy. 2018.13(4): 236-242. 14. Eun-Jeong Yu, Erika Hooker, Daniel T. Johnson, Mi Kyung Kwak, Kang Zou, Richard Luong, Yongfeng He, and Zijie Sun, LZTS2 and PTEN collaboratively regulate ß-catenin in prostatic tumorigenesis, Plos One. 2017.3.21, 12(3). 15. Rui Zhang, Jin Sun and Kang Zou*, Advances in Isolation Methods for Spermatogonial Stem Cells. Stem Cell Reviews and Reports. 2016,12(1):15-25. 16. Kang Zou, Lin Hou, Kejing Sun, Wenhai Xie and Ji Wu, Improved efficiency of female germline stem cell purification using fragilis-based magnetic bead sorting. Stem Cells and Development, 2011. 20(12): p. 2197-2204. 17. Yong Zhang, Zhaojuan Yang, Yunze Yang, Shuzeng Wang, Lingjun Shi, Wenhai Xie, Kejing Sun, Kang Zou, Lei Wang, Ji Xiong, Jie Xiang and Ji Wu, Production of transgenic mice by random recombination of targeted genes in female germline stem cells. Journal of Molecular Cell Biology, 2011. 3(2): 132-141. 18. Kang Zou, Zhe Yuan, Zhaojuan Yang, Huacheng Luo, Kejing Sun, Li Zhou, Jie Xiang, Lingjun Shi, Qingsheng Yu, Yong Zhang, Ruoyu Hou and Ji Wu, Production of offspring from a germline stem cell line derived from neonatal ovaries. Nature Cell Biology. 2009.11(5):631-636. 19. Ji Wu*, Yong Zhang, Gengeng Tian, Kang Zou, Clement M. Lee, Qingsheng Yu and Zhe Yuan, Short-type PB-cadherin promotes self-renewal of spermatogonial stem cells via multiple signaling pathways. Cellular Signalling, 2008. 20(6): 1052-1060. 实验室简介 (Germline Stem cells and Microenivronments Lab) 研究兴趣：本课题组致力于生殖干细胞的自我更新和分化机制研究，以及生殖干细胞的转化应用两个主要方向。 我们利用生殖干细胞体外培养系统和转基因模型小鼠为研究对象，从体外和体内两个角度来研究信号分子及微环境对生殖干细胞命运的调控作用，研究生殖干细胞自我更新和分化的生物学机制；同时利用独特的生殖干细胞技术，探究在生殖医学和畜牧学上的转化应用，包括利用干细胞模型在临床病例的研究，大动物的生殖干细胞的分离、体外培养系统，为医学和畜牧产业化提供技术支持。 研究内容简介： 生殖干细胞（germline stem cells）是生殖系统里负责配子发生的干细胞，具有自我更新和分化形成配子的潜力，其数量稀少，但有着重要的生物学意义。生殖干细胞在体内所处的微环境（microenvironment）对生殖干细胞命运的调控起决定性的作用。目前生殖干细胞在体内如何由静止状态（quiescence）向增殖(proliferation)或者分化（differentiation）状态的转换，以及相关的调控机制并不清楚。我们课题组通过从胚胎或者成体的生殖系统内分离生殖系干细胞并建立体外培养系统，以分子生物学、细胞生物学及干细胞移植等技术方法研究生殖干细胞在体外的生物特性及自我更新、分化机制，同时以转基因小鼠（overexpression, conventional and conditional KO）为模式动物，在体内分析和验证微环境对生殖干细胞的调控机制，探索生殖干细胞的形成、转化、自我更新和分化的调控机制。 基于对生殖干细胞的机制研究，我们也着力于将生殖干细胞与生物医学相结合，包括对生殖干细胞相关的疾病机制研究，以及生殖干细胞与临床医学的相结合。 此外，我们也致力于大动物生殖干细胞的研究。目前小鼠和人类的生殖干细胞的分离和培养技术已取得了成功，但大动物的生殖干细胞体外培养技术是一个瓶颈，如果能突破这一技术壁垒，对大动物转基因技术的操作、优良品种的选育、家畜生殖力的维持和保护都具有重要的意义。本课题组将参考小鼠精原干细胞的经验，摸索大动物的生殖干细胞的体外分离、鉴定和培养方法，试图建立一套大动物生殖干细胞的体外培养系统。 具体研究内容： 1 微环境对生殖干细胞自我更新和的分化的调控机制研究 2 生殖干细胞相关的疾病研究及临床应用 3 大动物生殖干细胞的分离、鉴定和体外培养 4 多能性干细胞的获得和应用 请点击如下链接，在Researchgate网站了解关于我们实验室更多的信息 (To find more details about our lab in the Researchgate website): https://www.researchgate.net/profile/Kang_Zou?ev=hdr_xprf |
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